ABSTRACT
ABSTRACT Major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of Alzheimer's disease have led to searching new control drugs. The present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. For such finality, five filamentous fungal species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were grown or not with DNA methyltransferases inhibitors (procainamide or hydralazine) and/or a histone deacetylase inhibitor (suberohydroxamic acid). Extracts from T. islandicus cultured or not with hydralazine inhibited Listeria monocytogenes growth in 57.66 ± 5.98% and 15.38 ± 1.99%, respectively. Increment in inhibition of acetylcholinesterase activity was observed for the extract from P. janthinellum grown with procainamide (100%), when compared to the control extract (39.62 ± 3.76%). Similarly, inhibition of acetylcholinesterase activity increased from 20.91 ± 3.90% (control) to 92.20 ± 3.72% when the tested extract was obtained from T. pinophilus under a combination of suberohydroxamic acid and procainamide. Concluding, increases in antimicrobial activity and acetylcholinesterase inhibition were observed when fungal extracts in the presence of DNA methyltransferases and/or histone deacetylase modulators were tested.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cholinesterase Inhibitors/pharmacology , Penicillium/chemistry , Talaromyces/chemistry , Acetylcholinesterase/chemistry , Acetylcholinesterase/metabolism , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/metabolism , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Cholinesterase Inhibitors/chemistry , Cholinesterase Inhibitors/metabolism , Chromatin/metabolism , Listeria monocytogenes/drug effects , Listeria monocytogenes/enzymology , Listeria monocytogenes/growth & development , Penicillium/metabolism , Talaromyces/metabolismABSTRACT
Com o proposito de avaliar a producao de lecitinase e a capacidade de adsorcao do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a producao de acido a partir de acucares aliada ao teste CAMP, correlacionando-se estes dados a capacidade de producao de ceratoconjuntivite em cobaia. As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorcao do corante e producao de lecitinase de 51,8 e 88,8 por cento, respectivamente, enquanto 80,8 e 100 por cento das culturas de L. innocua foram negativas para os referidos testes.